PHASE 3

the phase after success completed 100% somatic cell transfer.

PHASE 2

penetration to nukleus X02, by sucking pipet to release egg.

100% completed

PHASE 1

Materials

• Human Tissue: Pure human cells of one tissue type, from the individual who will be cloned.
• Human Tissue Culture Media: Media in which these human cells will grow and divide.
• Minimal Human Tissue Culture Media: Media in which cells will stop dividing, and enter a state of "quiescence" without dying.
• Laboratory supplies: Incubator, Sterile Hood, petri dishes, microscopes, and tools capable of removing and implanting cellular organelles, such as the nucleus, from one cell to another.
• Unfertilized human egg cells.
• Human Egg Cell growth media: Media where fertilized eggs will grow and divide.

Procedures

1. Grow the human cells to be cloned until you have a good supply.
2. Transfer the cells to minimal media. [For now, The Sheep Cloning Paper is a good reference for exactly how long.] This should allow the cells to live, but they should stop dividing and enter quiescence. This is likely the step in which the cells lose their differentiation, and revert to a more totipotent state.
3. When the cultured cells are in the quiescent state, get an unfertilized human egg cell. Remove the nucleus from this egg cell. Try to minimize damage done to this cell and discard the nucleus.
4. Take one of the quiescent cells in it's entirely, and implant it inside the coat around the egg (known as the zona pellucida) next to the egg itself.
5. Electroshock the egg. [For now, The Sheep Cloning Paper is probably a good reference for how much and how long to electroshock.] The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells. The rebooting of the human genetic program is believed to be initiated by the replacement of donor cell protien signals by egg cell protien signals, but the electroshock might assist in moving those protien signals across the nuclear membrane as well. Electroporation is a common technique for moving DNA molecules through a cellular membrane.
6. Repeat the last three steps as necessary until you have enough clones. Expect a lot of them not to survive because of cellular damage and other mishaps. Allow the embryos to grow and divide a few times in Human Egg Cell growth media.
7. Implant the embryos in human mothers where they will can be carried to term, and born normally.

Project X-Ivanna

First scheme, after 3 years preparation and long examination on many subject, i decide move to the next phase, to human egg.

This experimental project i named PROJECT X-Ivanna, the name Ivanna i take from my lovely wife, died on september 1990 of breast cancer.

start on jan 1993 with phase one.