research 01

Scientists have extracted stem cells from embryos in the past, hoping to one day use them as medicine. But they worried that the patient's body would reject stem cells taken from someone else. So for the past several years, various groups around the world have been trying to first create a clone, which would be a patient's exact biological match, then take stem cells from it.

Researchers have said they believe that some stem cells taken from embryos can be coaxed to become almost any type of cell in the human body, a characteristic known as "pluripotency." The stem cells in the Korean study differentiated into retinal, bone and other types of cells when implanted into mice.

Advanced Cell Technology, a private company in Worcester, Massachusetts, announced in a splashy Wired magazine cover story in January that it had cloned an embryo to the 16-cell stage. But the company apparently didn't extract stem cells from the embryo, and it wasn't clear how long the clone survived.

It's significant that the Korean research went through a stringent peer-review process in order to be published in Science, scientists say. Advanced Cell Technology has published just one study in an online scientific journal on its human-cloning experiments. In that study, the researchers -- including Cibelli, who worked there at the time -- achieved a clone with only six cells.

Human Clone Produces Stem Cells

In 1993 I conducted a poll called the International Bioethic Survey, which asked ordinary people about ethical dilemmas, and one of the questions was whether they would be for or against genetic enhancement in their children. An interesting finding was that in Thailand and China there is a lot of support for eugenic engineering. In western countries there seems to be a tolerant attitude towards modifying genes for medical reasons and yet a deep suspicion of doing it for enhancement purposes, but in Asia there isn’t that distinction. The philosophies of Buddhism, Hinduism and Confucianism don’t seem to lay down laws against trying to make ourselves better than we are.

One of the things that drove me and my co-authors to write our report on human cloning recently was the way the issue was being treated in the UN. In 2005 there was supposed to be a convention to ban reproductive cloning; it had unanimous support, but when some countries tried to ban embryo research as well we ended up with a split decision and a weak declaration. It was a missed chance and one of our main points in the report was that the UN has to do a better job at governing cloning.

The danger is that we’ll come to accept the idea of consumer children, or think that we can select our children from a supermarket catalogue – or we might clone in order to replace someone we've lost. That attitude is wrong because clones are inevitably different to the human being they came from. Even if there are a hundred clones from one person, every one of them is going to be an individual and has to be treated as such. The same is true even for a cyborg or any sort of future artificial intelligence creation. It may sound crazy, but they’re all beings.

Chinese team clone human embryos

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Thursday November 22nd 2007 Darryl Macer is a Unesco bioethicist who co-authored the recent UN study into reproductive cloning. In it he explains that

Human embryo clone, used for stem cell research. I started out studying biochemistry but became interested in bioethics after reading a work by Martin Luther King Junior, which was probably a strange way to be introduced. It was a book about social justice called Strength to Love, and it made me realise how important it is that society uses its knowledge to construct a just, ethical society.

I was also interested in the exchanges taking place between people who thought science was a good thing and those who were against it. There was a lot of early debate on genetic engineering and reproductive technology in the 1980s, and there seemed to be two different groups in society who couldn’t talk to each other. I felt it would be useful to become an intermediary between the technophiles and technophobes, those who like knowledge and those who dislike it.

I took a PhD in molecular biology because I wanted to understand the way scientists think, but the day I submitted my thesis I left the laboratory for good. I felt I couldn’t continue working as a lab scientist when there were so many interesting social questions to look at. I started to read philosophy and history of medicine full time.

Some people thought it was a bit of a waste of a laboratory scientist, but after a few years it became clear that bioethics was an interesting area in itself. I’ve never looked back. I still find that there are a lot of scientists working on the production of knowledge, but very few on how we should use it in society.

I am a Christian but I don’t find it hard to reconcile religion with science. As a scientist, I’ve worked with people of many different faiths and backgrounds – naturalists, Buddhists, Muslims and agnostics. There are basically two types of question: the scientific type, which is open to falsification, or disproving; and another, more important kind of question, like why is loving people better than hating them? I don’t really find any conflict between the two types. It’s wonderful to try to understand the natural world and use technology for the good of people and the environment, but that's just it: it has to be good, it has to stay within a moral framework.

PHASE 5

PHASE 4

Electroshock the egg. [For now, The Sheep Cloning Paper is probably a good reference for how much and how long to electroshock.] The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells. The rebooting of the human genetic program is believed to be initiated by the replacement of donor cell protien signals by egg cell protien signals, but the electroshock might assist in moving those protien signals across the nuclear membrane as well. Electroporation is a common technique for moving DNA molecules through a cellular membrane.

PHASE 3

the phase after success completed 100% somatic cell transfer.

PHASE 2

penetration to nukleus X02, by sucking pipet to release egg.

100% completed

PHASE 1

Materials

• Human Tissue: Pure human cells of one tissue type, from the individual who will be cloned.
• Human Tissue Culture Media: Media in which these human cells will grow and divide.
• Minimal Human Tissue Culture Media: Media in which cells will stop dividing, and enter a state of "quiescence" without dying.
• Laboratory supplies: Incubator, Sterile Hood, petri dishes, microscopes, and tools capable of removing and implanting cellular organelles, such as the nucleus, from one cell to another.
• Unfertilized human egg cells.
• Human Egg Cell growth media: Media where fertilized eggs will grow and divide.

Procedures

1. Grow the human cells to be cloned until you have a good supply.
2. Transfer the cells to minimal media. [For now, The Sheep Cloning Paper is a good reference for exactly how long.] This should allow the cells to live, but they should stop dividing and enter quiescence. This is likely the step in which the cells lose their differentiation, and revert to a more totipotent state.
3. When the cultured cells are in the quiescent state, get an unfertilized human egg cell. Remove the nucleus from this egg cell. Try to minimize damage done to this cell and discard the nucleus.
4. Take one of the quiescent cells in it's entirely, and implant it inside the coat around the egg (known as the zona pellucida) next to the egg itself.
5. Electroshock the egg. [For now, The Sheep Cloning Paper is probably a good reference for how much and how long to electroshock.] The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells. The rebooting of the human genetic program is believed to be initiated by the replacement of donor cell protien signals by egg cell protien signals, but the electroshock might assist in moving those protien signals across the nuclear membrane as well. Electroporation is a common technique for moving DNA molecules through a cellular membrane.
6. Repeat the last three steps as necessary until you have enough clones. Expect a lot of them not to survive because of cellular damage and other mishaps. Allow the embryos to grow and divide a few times in Human Egg Cell growth media.
7. Implant the embryos in human mothers where they will can be carried to term, and born normally.

Project X-Ivanna

First scheme, after 3 years preparation and long examination on many subject, i decide move to the next phase, to human egg.

This experimental project i named PROJECT X-Ivanna, the name Ivanna i take from my lovely wife, died on september 1990 of breast cancer.

start on jan 1993 with phase one.

one and half year of ivanna

my ivanna andara sentanu

1988/10/16 project X- ivanna - born