Thursday November 22nd 2007 Darryl Macer is a Unesco bioethicist who co-authored the recent UN study into reproductive cloning. In it he explains that

Human embryo clone, used for stem cell research. I started out studying biochemistry but became interested in bioethics after reading a work by Martin Luther King Junior, which was probably a strange way to be introduced. It was a book about social justice called Strength to Love, and it made me realise how important it is that society uses its knowledge to construct a just, ethical society.

I was also interested in the exchanges taking place between people who thought science was a good thing and those who were against it. There was a lot of early debate on genetic engineering and reproductive technology in the 1980s, and there seemed to be two different groups in society who couldn’t talk to each other. I felt it would be useful to become an intermediary between the technophiles and technophobes, those who like knowledge and those who dislike it.

I took a PhD in molecular biology because I wanted to understand the way scientists think, but the day I submitted my thesis I left the laboratory for good. I felt I couldn’t continue working as a lab scientist when there were so many interesting social questions to look at. I started to read philosophy and history of medicine full time.

Some people thought it was a bit of a waste of a laboratory scientist, but after a few years it became clear that bioethics was an interesting area in itself. I’ve never looked back. I still find that there are a lot of scientists working on the production of knowledge, but very few on how we should use it in society.

I am a Christian but I don’t find it hard to reconcile religion with science. As a scientist, I’ve worked with people of many different faiths and backgrounds – naturalists, Buddhists, Muslims and agnostics. There are basically two types of question: the scientific type, which is open to falsification, or disproving; and another, more important kind of question, like why is loving people better than hating them? I don’t really find any conflict between the two types. It’s wonderful to try to understand the natural world and use technology for the good of people and the environment, but that's just it: it has to be good, it has to stay within a moral framework.

PHASE 5

PHASE 4

Electroshock the egg. [For now, The Sheep Cloning Paper is probably a good reference for how much and how long to electroshock.] The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells. The rebooting of the human genetic program is believed to be initiated by the replacement of donor cell protien signals by egg cell protien signals, but the electroshock might assist in moving those protien signals across the nuclear membrane as well. Electroporation is a common technique for moving DNA molecules through a cellular membrane.

PHASE 3

the phase after success completed 100% somatic cell transfer.

PHASE 2

penetration to nukleus X02, by sucking pipet to release egg.

100% completed

PHASE 1

Materials

• Human Tissue: Pure human cells of one tissue type, from the individual who will be cloned.
• Human Tissue Culture Media: Media in which these human cells will grow and divide.
• Minimal Human Tissue Culture Media: Media in which cells will stop dividing, and enter a state of "quiescence" without dying.
• Laboratory supplies: Incubator, Sterile Hood, petri dishes, microscopes, and tools capable of removing and implanting cellular organelles, such as the nucleus, from one cell to another.
• Unfertilized human egg cells.
• Human Egg Cell growth media: Media where fertilized eggs will grow and divide.

Procedures

1. Grow the human cells to be cloned until you have a good supply.
2. Transfer the cells to minimal media. [For now, The Sheep Cloning Paper is a good reference for exactly how long.] This should allow the cells to live, but they should stop dividing and enter quiescence. This is likely the step in which the cells lose their differentiation, and revert to a more totipotent state.
3. When the cultured cells are in the quiescent state, get an unfertilized human egg cell. Remove the nucleus from this egg cell. Try to minimize damage done to this cell and discard the nucleus.
4. Take one of the quiescent cells in it's entirely, and implant it inside the coat around the egg (known as the zona pellucida) next to the egg itself.
5. Electroshock the egg. [For now, The Sheep Cloning Paper is probably a good reference for how much and how long to electroshock.] The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells. The rebooting of the human genetic program is believed to be initiated by the replacement of donor cell protien signals by egg cell protien signals, but the electroshock might assist in moving those protien signals across the nuclear membrane as well. Electroporation is a common technique for moving DNA molecules through a cellular membrane.
6. Repeat the last three steps as necessary until you have enough clones. Expect a lot of them not to survive because of cellular damage and other mishaps. Allow the embryos to grow and divide a few times in Human Egg Cell growth media.
7. Implant the embryos in human mothers where they will can be carried to term, and born normally.

Project X-Ivanna

First scheme, after 3 years preparation and long examination on many subject, i decide move to the next phase, to human egg.

This experimental project i named PROJECT X-Ivanna, the name Ivanna i take from my lovely wife, died on september 1990 of breast cancer.

start on jan 1993 with phase one.